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Specifications With the click of one button, NGSengine performs locus assignment, read alignment, read phasing, and genotyping. NGS-based RNA-Seq is a powerful method that enables researchers to break through the inefficiency and expense of legacy technologies such as microarrays.
Microarray gene expression measurement is limited by noise at the low end and signal saturation at the high end. In contrast, next-gen sequencing quantifies discrete, digital sequencing read counts, offering a broader dynamic range. Compare Arrays vs. Targeted sequencing allows you to sequence a subset of genes or specific genomic regions of interest, efficiently and cost-effectively focusing the power of NGS. NGS is highly scalable, allowing you to tune the level of resolution to meet experimental needs.
Choose whether to do a shallow scan across multiple samples, or sequence at greater depth with fewer samples to find rare variants in a given region. Next-generation sequencing is uniquely positioned in an infectious disease surveillance and outbreak model. Learn which NGS methods are recommended for detecting and characterizing SARS-CoV-2 and other respiratory pathogens, tracking transmission, studying co-infection, and investigating viral evolution.
Illumina sequencing utilizes a fundamentally different approach from the classic Sanger chain-termination method. It leverages sequencing by synthesis SBS technology — tracking the addition of labeled nucleotides as the DNA chain is copied — in a massively parallel fashion. Next-generation sequencing generates masses of DNA sequencing data, and is both less expensive and less time-consuming than traditional Sanger sequencing.
This detailed overview of Illumina sequencing describes the evolution of genomic science, major advances in sequencing technology, key methods, the basics of Illumina sequencing chemistry, and more.
Researchers use single-cell techniques to study cancer microenvironments, to elucidate gene expression patterns and gain insights into drug resistance and metastasis. Whole-exome and transcriptome sequencing prove beneficial in uncovering mutations and pathways associated with rare genetic diseases. The resources below offer valuable guidance to scientists who are considering purchasing a next-generation sequencing system.
Learn about read length, coverage, quality scores, and other experimental considerations to help you plan your sequencing run. Use our interactive tools to help you create a custom NGS protocol or select the right products and methods for your project. Start Planning Experiments. Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place.
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